choline and arsenocholine [SW|ABC transporter] (ATP-binding protein)
function
compatible solute transport
product
choline and arsenocholine [SW|ABC transporter] (ATP-binding protein)
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.4|Uptake of compatible solutes for osmoprotection][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
3,462,105 → 3,463,250
The protein
Catalyzed reaction/ biological activity
uptake of choline and arsenocholine [pubmed|29159878]Protein family
[SW|ABC transporter] family (according to Swiss-Prot)Paralogous protein(s)
[protein|75DB6231129C28C5D3791BA43A1261CD46A861E8|OpuCA][SW|Domains]
2 [SW|CBS domain]s (256-314, 316-374)Structure
[PDB|2IT1] (from Pyrococcus horikoshii, 43% identity)[SW|Localization]
associated to the membrane (via [protein|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|OpuBB]-[protein|1532EC3D741C5AB3D0B642741218FAE00AD460FA|OpuBD]) [Pubmed|10092453]Expression and Regulation
Operons
genes
[gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]
description
[Pubmed|10216873]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10216873], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]: repression, (transcriptional roadblock) [Pubmed|32849357,22408163], in [regulon|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR regulon][protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR]: repression, (sterical interference with RNA polymerase access) [Pubmed|32849357,23960087], in [regulon|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR regulon][protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]regulation
induced by choline ([protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]) [Pubmed|22408163]expression increases with increasing salinity [Pubmed|32849357]additional information
[gene|6FCF6F71C5A8B2345F42BD0C3345C520FD40358B|S1290] acts as [SW|ncRNA|antisense RNA] for the [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD] operon [PubMed|,32373088,20525796]expression starts with a delay after osmotic upshift due to transcriptional interference of [SW|RNA polymerase] complexes driving synthesis of the converging opuB and [gene|6FCF6F71C5A8B2345F42BD0C3345C520FD40358B|S1290] mRNAs [pubmed|32373088]view in new tabBiological materials
Mutant
BKE33730 (Δ[gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACATCGCACCTCCAAAA, downstream forward: _UP4_TAGGGAGGGGCGATAAGTCABKK33730 (Δ[gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACATCGCACCTCCAAAA, downstream forward: _UP4_TAGGGAGGGGCGATAAGTCAExpression vector
pGP2938: (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s labpGP3013: (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s labReferences
Reviews
Original publications
10092453,9925583,10216873,21296969,23960087,22408163,23646920,29159878